We’re currently running a series of tests on serum samples to quantify specific protein biomarkers, and while ELISA remains our go-to method due to its sensitivity and specificity, the overwhelming number of kit options makes selection pretty confusing. Some kits differ a lot in terms of detection ranges, sample volume required, and even cross-reactivity rates. We’re trying to avoid unreliable results that could come from kits with suboptimal antibody specificity or low reproducibility. Does anyone have a structured approach to evaluating these kits before committing to one? Are there parameters beyond just the datasheet and IFU (instructions for use) that are worth considering during the decision-making process?